'Yersinia pseudotuberculosis' mntH functions in intracellular manganese accumulation, which is essential for virulence and survival in cells expressing functional Nramp1

Champion, Olivia L., Karlyshev, Andrey, Cooper, A. M., Ford, Donna C., Wren, Brendan W., Duffield, Melanie, Oyston, Petra C. F. and Titball, Richard W. (2011) 'Yersinia pseudotuberculosis' mntH functions in intracellular manganese accumulation, which is essential for virulence and survival in cells expressing functional Nramp1. Microbiology, 157(4), pp. 1115-1122. ISSN (print) 1350-0872

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Abstract

Manganese has an important yet undefined role in the virulence of many bacterial pathogens. In this study we confirm that a null mutation in Yersinia pseudotuberculosis mntH reduces intracellular manganese accumulation. An mntH mutant was susceptible to killing by reactive oxygen species when grown under manganese-limited conditions. The mntH mutant was defective in survival and growth in macrophages expressing functional Nramp1, but in macrophages deficient in Nramp the bacteria were able to survive and replicate. In Galleria mellonella, the mntH mutant was attenuated. Taken together, these data suggest a role for manganese in Y. pseudotuberculosis during macrophage intracellular survival, protecting the bacteria from the antimicrobial products released during the respiratory burst.

Item Type: Article
Research Area: Agriculture, veterinary and food science
Allied health professions and studies
Biological sciences
Infection and immunology
Faculty, School or Research Centre: Faculty of Science (until 2011) > School of Life Sciences
Related URLs:
Depositing User: Andrey Karlyshev
Date Deposited: 20 Sep 2011 08:18
Last Modified: 02 Jan 2013 15:10
URI: http://eprints.kingston.ac.uk/id/eprint/20186

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