Improved assay for R(-)-apomorphine with application to clinical pharmacokinetic studies in Parkinson's disease

Ingram, Wendy M., Priston, Melanie J. and Sewell, Graham J. (2006) Improved assay for R(-)-apomorphine with application to clinical pharmacokinetic studies in Parkinson's disease. Journal of Chromatography B, 831(1-2), pp. 1-7. ISSN (print) 1570-0232

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Abstract

A high performance liquid chromatographic assay for the quantitative determination of apomorphine in human plasma is described. Sample clean-up and concentration was optimised using solid-phase extraction on C18 cartridges, enabling rapid and sensitive determination of apomorphine and potential metabolites. The limit of apomorphine quantification, using fluorescence detection, was 0.5 ng/mL. The assay was stability-indicating, and allowed the detection of analytes in the presence of commonly co-administered anti-Parkinsonian drugs. Apomorphine was stable in frozen plasma containing 0.14% (w/v) ascorbic acid for 98 days, and through four freeze-thaw cycles. The assay has been used in clinical pharmacokinetic studies of apomorphine in patients with Parkinson's disease, and in preliminary studies of novel apomorphine delivery devices in volunteers.

Item Type: Article
Uncontrolled Keywords: apomorphine, Parkinson's disease, hplc, solid phase extraction, plasma, pharmacokinetics, performance liquid-chromatography, apomorphine enantiomers, R-apomorphine, plasma, extraction, infusion
Research Area: Allied health professions and studies
Faculty, School or Research Centre: Faculty of Science (until 2011) > School of Pharmacy and Chemistry
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Depositing User: Sara Burnett
Date Deposited: 02 Jul 2007
Last Modified: 14 Jun 2011 12:58
URI: http://eprints.kingston.ac.uk/id/eprint/1519

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