Improved assay for R(-)-apomorphine with application to clinical pharmacokinetic studies in Parkinson's disease

Ingram, Wendy M., Priston, Melanie J. and Sewell, Graham J. (2006) Improved assay for R(-)-apomorphine with application to clinical pharmacokinetic studies in Parkinson's disease. Journal of Chromatography B, 831(1-2), pp. 1-7. ISSN (print) 1570-0232

Official URL: http://dx.doi.org/10.1016/j.jchromb.2005.11.005

Abstract

A high performance liquid chromatographic assay for the quantitative determination of apomorphine in human plasma is described. Sample clean-up and concentration was optimised using solid-phase extraction on C18 cartridges, enabling rapid and sensitive determination of apomorphine and potential metabolites. The limit of apomorphine quantification, using fluorescence detection, was 0.5 ng/mL. The assay was stability-indicating, and allowed the detection of analytes in the presence of commonly co-administered anti-Parkinsonian drugs. Apomorphine was stable in frozen plasma containing 0.14% (w/v) ascorbic acid for 98 days, and through four freeze-thaw cycles. The assay has been used in clinical pharmacokinetic studies of apomorphine in patients with Parkinson's disease, and in preliminary studies of novel apomorphine delivery devices in volunteers.

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