Plows, Louise D., Cook, Richard T., Davies, Angela J. and Walker, Anthony J. (2006) Phagocytosis by Lymnaea stagnalis haemocytes: a potential role for phosphatidylinositol 3-kinase but not protein kinase A. Journal of Invertebrate Pathology, 91(1), pp. 74-77. ISSN (print) 0022-2011Full text not available from this archive.
The molecular events that regulate phagocytosis, an important innate immune response, in invertebrate defence cells (haemocytes) are poorly understood. Lymnaea stagnalis haemocytes were used as a model to elucidate the role of cell signalling pathways in phagocytosis by molluscan defence cells. The phosphatidylinositol 3-kinase (PI3-K) inhibitor, LY294002, significantly impaired haemocyte phagocytic activity in a dose-responsive manner with 10 microM LY294002 reducing internalization of fluorescent-conjugated Escherichia coli by 62% (P < or = 0.001). In contrast, the protein kinase A (PKA) inhibitor KT5720 was without effect. Therefore, PI3-K, but not PKA, appears to control phagocytosis by haemocytes in these gastropod molluscs.
|Additional Information:||This work was supported by the Royal Society and Kingston University|
|Research Area:||Biological sciences|
|Faculty, School or Research Centre:||Faculty of Science (until 2011) > School of Life Sciences|
|Depositing User:||Automatic Import Agent|
|Date Deposited:||22 Feb 2010 11:11|
|Last Modified:||30 Sep 2010 10:52|
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