A novel medium devoid of ruminant peptone for high yield growth of Mycoplasma ovipneumoniae

Patel, Hiren, Mackintosh, David, Ayling, Roger D., Nicholas, Robin A. J. and Fielder, Mark D. (2008) A novel medium devoid of ruminant peptone for high yield growth of Mycoplasma ovipneumoniae. Veterinary Microbiology, 127(3-4), pp. 309-314. ISSN (print) 0378-1135

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Abstract

Mycoplasma ovipneumoniae is considered an emerging veterinary pathogen causing pneumonia in sheep and goats worldwide. Currently it has not been possible to define a growth medium that yields the maximum growth of M. ovipneumoniae within a short incubation period. Growth yields of M. ovipneumoniae in Eaton's medium are variable and not as consistently high as those seen with other Mycoplasma spp. This study investigated the ability of different M. ovipneumoniae field strains to grow in various media formulations, where PPLO broth was replaced by a vegetable protein source, and comparisons were made in terms of strain viability in Eaton's medium. Studies were also conducted to determine the optimal carbohydrate source for use in the M. ovipneumoniae medium. Generally, it was found that different strains showed good growth in all media tested, with growth yields at 24h in TSB-1 medium higher than those observed with Eaton's medium. Growth yields reached 10(8) to 10(9)cfu ml(-1) within 24h for particular field strains, with all strains achieving this growth level within 48-72h.

Item Type: Article
Uncontrolled Keywords: Mycoplasma ovipneumoniae, culture, diagnosis, emerging veterinary pathogen, vaccine, respiratory-infections, sheep, pneumonia, lambs
Research Area: Agriculture, veterinary and food science
Faculty, School or Research Centre: Faculty of Science (until 2011) > School of Life Sciences
Services and Administration > Vice-Chancellor's Office
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Depositing User: Automatic Import Agent
Date Deposited: 11 Feb 2010 13:26
Last Modified: 07 Oct 2011 16:23
URI: http://eprints.kingston.ac.uk/id/eprint/7028

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