Specific tyrosine phosphorylation induced in Schistosoma mansoni miracidia by haemolymph from schistosome susceptible, but not resistant, Biomphalaria glabrata

Walker, A.J. and Rollinson, David (2008) Specific tyrosine phosphorylation induced in Schistosoma mansoni miracidia by haemolymph from schistosome susceptible, but not resistant, Biomphalaria glabrata. Parasitology, 135(3), pp. 337-345. ISSN (print) 0031-1820

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Abstract

Molecular interplay during snail-schistosome interactions is poorly understood and there is much to discover concerning the effect of snail host molecules on molecular processes in schistosomes. Using the Biomphalaria glabrata - Schistosoma mansoni host-parasite system, the effects of exposure to haemolymph, derived from schistosome-resistant and susceptible snail strains, on protein tyrosine phosphorylation in miracidia have been investigated. Western blotting revealed several tyrosine phosphorylated proteins in this larval stage. Exposure of miracidia to haemolymph from susceptible snails for 60 min resulted in a striking, 5-fold, increase in the tyrosine phosphorylation of a 56 kDa (p56) S. mansoni protein. In contrast, haemolymph from resistant snails had little effect on protein tyrosine phosphorylation levels in miracidia. Confocal microscopy revealed that tyrosine phosphorylation was predominantly associated with proteins present in the tegument. Finally, treatment of miracidia with the tyrosine kinase inhibitor genistein significantly impaired their development into primary sporocysts. The results open avenues for research that focus on the potential importance of phospho-p56 to the outcome of schistosome infection in snails, and the significance of protein tyrosine kinase-mediated signalling events to the transformation of S. mansoni larvae.

Item Type: Article
Uncontrolled Keywords: schistosoma mansoni, biomphalaria glabrata, miracidia, tyrosine kinase, protein phosphorylation, molluscan defence, haemolymph, host-parasite interactions, epidermal-growth-factor, lymnaea-stagnalis, gene-expression, protein-phosphorylation, defense cells, kinase, identification, involvement, activation, sporocysts
Research Area: Biological sciences
Faculty, School or Research Centre: Faculty of Science (until 2011)
Faculty of Science (until 2011) > School of Life Sciences
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Depositing User: Automatic Import Agent
Date Deposited: 26 May 2010 10:49
Last Modified: 26 Apr 2013 12:59
URI: http://eprints.kingston.ac.uk/id/eprint/7022

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