Analytical and physicochemical characterisation of the senile cataract drug dipeptide [Beta]-alanyl-L-histidine (carnosine)

Abdelkader, Hamdy, Swinden, Julian, Pierscionek, Barbara K and Alany, Raid G (2015) Analytical and physicochemical characterisation of the senile cataract drug dipeptide [Beta]-alanyl-L-histidine (carnosine). Journal of Pharmaceutical and Biomedical Analysis, 114, pp. 241-246. ISSN (print) 0731-7085

Full text available as:
[img] Text
Alany-R-31945.pdf - Accepted Version
Available under License Creative Commons Attribution Non-commercial No Derivatives.

Download (254kB)

Abstract

This study presents a simple but sensitive HPLC chromatographic method with a stability-indicating assay for determination and physicochemical characterisation of L-carnosine, a promising senile cataract prophylactic agent. Chromatographic analysis was conducted using a reverse phase (RP)-HPLC system and an isocratic mobile phase of 98% v/v trifluoroacetic acid (0.1% v/v) and 2% v/v acetonitrile with detection at 220nm. L-carnosine was subjected to stress conditions to force its degradation using chemical and thermal agents and was subsequently detected from its degradation products using ESI-MS. The lipophilicity of the drug and 1:1 drug to phospholipid complex (PC) mol/mol was determined by estimating the partition coefficient (P). Lipophilicity was greatly enhanced when L-carnosine was formulated as a phospholipid complex using the solvent evaporation method. L-carnosine-phospholipid complex could be a promising approach for effective delivery to the human lens as offers a potential novel treatment for senile cataract.

Item Type: Article
Research Area: Chemistry
Faculty, School or Research Centre: Faculty of Science, Engineering and Computing
Faculty of Science, Engineering and Computing > School of Pharmacy and Chemistry
Related URLs:
Depositing User: Automatic Import Agent
Date Deposited: 06 Jul 2015 09:22
Last Modified: 13 Jun 2016 13:23
URI: http://eprints.kingston.ac.uk/id/eprint/31945

Actions (Repository Editors)

Item Control Page Item Control Page