Manipulation of systemic oxygen flux by acute exercise and normobaric hypoxia: implications for reactive oxygen species generation

Davison, Gareth W., Mogan, Rhian M., Hiscock, Natalie, Garcia, Juan M., Grace, Fergal, Boisseau, Natalie, Davies, Bruce, Castell, Linda, McEneny, Jane, Young, Ian S., Hullin, David, Ashton, Tony and Bailey, Damian M. (2005) Manipulation of systemic oxygen flux by acute exercise and normobaric hypoxia: implications for reactive oxygen species generation. Clinical Science, 110(1), pp. 113-141. ISSN (print) 0143-5221

Full text not available from this archive.

Abstract

Maximal exercise in normoxia results in oxidative stress due to an increase in free radical production. However, the effect of a single bout of moderate aerobic exercise performed in either relative or absolute normobaric hypoxia on free radical production and lipid peroxidation remains unknown. To examine this, we randomly matched {according to their normobaric normoxic O2peak [peak O2 (oxygen uptake)]} and assigned 30 male subjects to a normoxia (n=10), a hypoxia relative (n=10) or a hypoxia absolute (n=10) group. Each group was required to exercise on a cycle ergometer at 55% of O2peak for 2 h double-blinded to either a normoxic or hypoxic condition [FiO2 (inspired fraction of O2)=0.21 and 0.16 respectively]. ESR (electron spin resonance) spectroscopy in conjunction with ex vivo spin trapping was utilized for the direct detection of free radical species. The main findings show that moderate intensity exercise increased plasma-volume-corrected free radical and lipid hydroperoxide concentration (pooled rest compared with exercise data, P<0.05); however, there were no selective differences between groups (state×group interaction, P>0.05). The delta change in free radical concentration was moderately correlated with systemic O2 (r2=0.48, P<0.05). The hyperfine coupling constants recorded from the ESR spectra [aN=13.8 Gauss, and aHb=1.9 Gauss; where 1 Gauss=10-4 T (telsa)] are suggestive of oxygen-centred free radical species formed via the decomposition of lipid hydroperoxides. Peripheral leucocyte and neutrophil cells and total CK (creatine kinase) activity all increased following sustained exercise (pooled rest compared with exercise data, P<0.05), but no selective differences were observed between groups (state×group interaction, P>0.05). We conclude that a single bout of moderate aerobic exercise increases secondary free radical species. There is also evidence of exercise-induced muscle damage, possibly caused by the increase in free radical generation.

Item Type: Article
Research Area: Allied health professions and studies
Faculty, School or Research Centre: Faculty of Health and Social Care Sciences
Depositing User: Katrina Clifford
Date Deposited: 22 May 2008
Last Modified: 14 May 2009 14:50
URI: http://eprints.kingston.ac.uk/id/eprint/2905

Actions (Repository Editors)

Item Control Page Item Control Page