Identification of a major causative agent of human cercarial dermatitis, Trichobilharzia franki (Muller and Kimmig 1994), in southern England and its evolutionary relationships with other European populations.

Lawton, Scott P., Lim, Rivka M., Dukes, Juliet P., Cook, Richard T., Walker, Anthony J. and Kirk, Ruth S. (2014) Identification of a major causative agent of human cercarial dermatitis, Trichobilharzia franki (Muller and Kimmig 1994), in southern England and its evolutionary relationships with other European populations. Parasites & Vectors, 7, p. 277. ISSN (online) 1756-3305

Full text available as:
[img]
Preview
Text
Lawton-S-28686.pdf - Published Version
Available under License Creative Commons Attribution.

Download (826kB) | Preview

Abstract

Background Trichobilharzia is the most species rich and widely distributed genus of schistosomes and is known throughout Europe and North America as an agent of human cercarial dermatitis. The disease is caused by an acute allergic reaction in the skin that develops as a consequence of repeated contact with water containing schistosomatid cercariae. However, despite historical outbreaks of the disease, there are no published records of accurately identified Trichobilharzia species from the UK. Methods Two hundred Radix auricularia (L.) were sampled from a recreational fishing lake in Hampshire and emerging schistosomatid cercariae were collected for microscopy and DNA extraction. General morphological description of the cercariae was performed, alongside sequencing and phylogenetic analysis of the 28S ribosomal DNA for accurate species identification as well as comparisons of ITS1 in order to identify evolutionary affinities with other European populations. All molecular comparisons were performed using published sequences. Results The phylogenetic analysis of 28S sequences identified the cercariae as Trichobilharzia franki. Two unique British ITS1 haplotypes were identified which were most closely related to haplotypes of T. franki populations from France. Haplotype network analysis indicated the mixing of T. franki populations throughout Europe. It is suggested that parasite distribution is the probable result of the movement of migratory waterfowl. Conclusions This is the first accurate record of T. franki in the UK. The movement of T. franki with waterfowl could pose a considerable human health risk, as in mainland Europe, and signifies T. franki-associated human cercarial dermatitis as a re-emerging disease in the UK.

Item Type: Article
Uncontrolled Keywords: Trichobilharzia franki, cercarial dermatitis, UK, re-emerging disease, 28s ribosomal DNA, ITS1 haplotypes
Research Area: Allied health professions and studies
Faculty, School or Research Centre: Faculty of Science, Engineering and Computing
Faculty of Science, Engineering and Computing > School of Life Sciences
Related URLs:
Depositing User: Automatic Import Agent
Date Deposited: 20 Jan 2015 11:37
Last Modified: 24 Feb 2015 15:26
URI: http://eprints.kingston.ac.uk/id/eprint/28686

Actions (Repository Editors)

Item Control Page Item Control Page