Holloway, Andrew, Mueller-Harvey, Irene, Gould, Simon W. G., Fielder, Mark D., Naughton, Declan P. and Kelly, Alison F. (2012) The effect of copper(ii), iron(ii) sulphate, and vitamin C combinations on the weak antimicrobial activity of (+)-catechin against Staphylococcus aureus and other microbes. Metallomics : integrated biometal science, 4(12), pp. 1280-1286. ISSN (print) 1756-5901Full text not available from this archive.
Few attempts have been made to improve the activity of plant compounds with low antimicrobial efficacy. (+)-Catechin, a weak antimicrobial tea flavanol, was combined with putative adjuncts and tested against different species of bacteria. Copper(ii) sulphate enhanced (+)-catechin activity against Pseudomonas aeruginosa but not Staphylococcus aureus, Proteus mirabilis or Escherichia coli. Attempts to raise the activity of (+)-catechin against two unresponsive species, S. aureus and E. coli, with iron(ii) sulphate, iron(iii) chloride, and vitamin C, showed that iron(ii) enhanced (+)-catechin against S. aureus, but not E. coli; neither iron(iii) nor combined iron(ii) and copper(ii), enhanced (+)-catechin activity against either species. Vitamin C enhanced copper(ii) containing combinations against both species in the absence of iron(ii). Catalase or EDTA added to active samples removed viability effects suggesting that active mixtures had produced H(2)O(2)via the action of added metal(ii) ions. H(2)O(2) generation by (+)-catechin plus copper(ii) mixtures and copper(ii) alone could account for the principal effect of bacterial growth inhibition following 30 minute exposures as well as the antimicrobial effect of (+)-catechin-iron(ii) against S. aureus. These novel findings about a weak antimicrobial flavanol contrast with previous knowledge of more active flavanols with transition metal combinations. Weak antimicrobial compounds like (+)-catechin within enhancement mixtures may therefore be used as efficacious agents. (+)-Catechin may provide a means of lowering copper(ii) or iron(ii) contents in certain crop protection and other products.
|Research Area:||Allied health professions and studies
|Faculty, School or Research Centre:||Faculty of Science, Engineering and Computing
Faculty of Science, Engineering and Computing > School of Life Sciences
|Depositing User:||Automatic Import Agent|
|Date Deposited:||13 Nov 2012 15:21|
|Last Modified:||08 Jan 2013 11:29|
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