Enzymatic digestion and selective quantification of underivatised [delta]9-tetrahydrocannabinol and cocaine in human hair using gas chromatography-mass spectrometry.

Breidi, Salah Eddine, Barker, James, Petroczi, Andrea and Naughton, Declan (2012) Enzymatic digestion and selective quantification of underivatised [delta]9-tetrahydrocannabinol and cocaine in human hair using gas chromatography-mass spectrometry. Journal of Analytical Methods in Chemistry, ISSN (print) 2090-8865

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Abstract

Gas chromatography-mass spectrometric (GC-MS) methods for drug analysis routinely employ derivatising reagents. The aim of this paper was to develop a method for the analysis of two recreational drugs, delta-9-tetrahydrocannabinol ([delta](9)-THC) and cocaine in hair samples using GC-MS, without prior derivatisation, thus allowing the sample to be reanalysed in its original form. An enzymatic digestion technique was also developed. Ten hair samples, that were known positive for either [delta](9)-THC and/or cocaine, were enzymatically digested, extracted, and then analysed by GC-MS. All samples measured contained [delta](9)-THC and one sample contained cocaine. The limits of detection (LOD) and quantification (LOQ) were 0.02 ng/mg and 0.05 ng/mg, respectively, for cocaine and 0.015 ng/mg and 0.02 ng/mg, respectively, for [delta](9)-THC. The wide detection window, ease of direct analysis by GC-MS, lower detection limits of underivatised samples, and the stability of drugs using this technique may offer an improved method of analysis.

Item Type: Article
Uncontrolled Keywords: solid-phase microextraction, drug-use, delta(9)-tetrahydrocannabinol, cannabinoids, samples, abuse, derivatization, cannabidiol, consumption, license
Research Area: Allied health professions and studies
Faculty, School or Research Centre: Faculty of Science, Engineering and Computing
Faculty of Science, Engineering and Computing > School of Life Sciences
Faculty of Science, Engineering and Computing > School of Pharmacy and Chemistry
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Depositing User: Declan Naughton
Date Deposited: 13 Feb 2012 16:23
Last Modified: 24 Aug 2012 14:24
URI: http://eprints.kingston.ac.uk/id/eprint/22221

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