Transgenic 'Arabidopsis' leaf tissue expressing a modified oryzacystatin shows resistance to the field slug 'Deroceras reticulatum' (Muller).

Walker, Anthony J., Urwin, Peter E., Atkinson, Howard J., Brain, Philip, Glen, David M. and Shewry, Peter R. (1999) Transgenic 'Arabidopsis' leaf tissue expressing a modified oryzacystatin shows resistance to the field slug 'Deroceras reticulatum' (Muller). Transgenic Research, 8(2), pp. 95-103. ISSN (print) 0962-8819

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Abstract

Transgenic 'Arabidopsis thaliana' has been developed which expresses the oryzacystatin mutant OC-I delta 86, which is an inhibitor of the major proteinase present in the digestive gland of the slug, 'Deroceras reticulatum'. When fed on leaf tissue from plants expressing this inhibitor the growth of juvenile slugs was significantly reduced by 31% compared with those feeding on control leaf tissue. Furthermore, while surviving slugs did not individually consume less when feeding on leaf tissue expressing OC-I delta 86, the total amount of leaf tissue eaten was 50% less, due to reduced survival of slugs. The synthetic cysteine proteinase inhibitors E-64 and leupeptin also significantly reduced slug weight gain (by at least 40%) and digestive gland cysteine proteinase activity when administered in an artificial diet, indicating that their antimetabolic effects are due to direct inhibition of gut proteolytic activity. These results suggest that transgenic crop plants expressing phytocystatins could be used to suppress the growth rates of slug populations in the field.

Item Type: Article
Additional Information: This work was supported by the Biotechnology and Biological Sciences Research Council.
Uncontrolled Keywords: transgenic plants, crop protection, slug, mollusc, proteinase inhibitor, Arabidopsis thaliana
Research Area: Biological sciences
Faculty, School or Research Centre: Faculty of Science (until 2011) > School of Life Sciences
Related URLs:
Depositing User: Tony Walker
Date Deposited: 25 Mar 2011 12:14
Last Modified: 25 Mar 2011 12:14
URI: http://eprints.kingston.ac.uk/id/eprint/16359

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