Mitogenic activity of insulin in the culture of a trypanosome: duration and dose response

Mackintosh, David, Coleman, Kim and Davies, Angela J. (2003) Mitogenic activity of insulin in the culture of a trypanosome: duration and dose response. Cell Biology International, 27(1), pp. 75-78. ISSN (print) 1065-6995

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Abstract

The effects of insulin on the in vitro growth characteristics of Trypanosoma granulosum were investigated. The insulin growth stimulus had a rapid onset with little or no lag phase but was relatively short lived, growth peaking typically on day 3 or 4 of culture. This contrasted with medium containing 10% foetal calf serum, normally used for in vitro culture of this trypanosome, which after a 3 day lag stimulated sustained log-phase growth. Insulin demonstrated a biphasic dose response with maximum growth typically occurring at 3-10 microg/ml and a much reduced effect at higher concentrations. These findings suggest a possible role for host insulin and/or trypanosomal insulin-like molecules in regulating parasite growth in vivo.

Item Type: Article
Research Area: Allied health professions and studies
Faculty, School or Research Centre: Faculty of Science (until 2011) > School of Life Sciences
Faculty of Science (until 2011)
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Depositing User: Sara Burnett
Date Deposited: 25 Jun 2007
Last Modified: 22 Mar 2011 16:40
URI: http://eprints.kingston.ac.uk/id/eprint/1486

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