A rapid LC-MS method for determination of plasma anion profiles of acidotic patients

McKinnon, William, Lord, Gwyn A, Forni, Lui G, Peron, Jean-Marie R and Hilton, Philip J (2006) A rapid LC-MS method for determination of plasma anion profiles of acidotic patients. Journal of Chromatography B, 833(2), pp. 179-185. ISSN (print) 1570-0232

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In metabolic acidosis, the concentrations of anions associated with intermediary metabolism are increased and can make a significant contribution to the observed acidosis. Here we describe a method for the rapid determination of the plasma ultrafiltrate profile of these anions using liquid chromatography coupled to electrospray ionisation mass spectrometry (LC/ESI-MS). The ultrafiltrate from patients with acidosis resulting from various causes were examined and the results compared to control values. Using the LC/ESI-MS method described, a unique plasma ultrafiltrate anion profile was obtained for each of the groups studied that provides rapid diagnosis of the type of underlying acidosis.

Item Type: Article
Uncontrolled Keywords: acidosis, patients, plasma, ultrafiltrate, Krebs cycle, anion gap, intermediatry metabolism, liquid chromatography, mass spectrometry, organic-acids, metabolic-acidosis, mass-spectrometry, renal-failure, chromatography, cycle
Research Area: Chemistry
Other laboratory based clinical subjects
Faculty, School or Research Centre: Faculty of Science (until 2011) > School of Pharmacy and Chemistry
Related URLs:
Depositing User: Jean-Marie Peron
Date Deposited: 12 Nov 2010 10:00
Last Modified: 25 Nov 2011 10:06
DOI: https://doi.org/10.1016/j.jchromb.2006.01.025
URI: http://eprints.kingston.ac.uk/id/eprint/7384

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