Enrichment of immunoregulatory proteins in the biomolecular corona of nanoparticles within human respiratory tract lining fluid

Kumar, Abhinav, Bicer, Elif Melis, Morgan, Anna Babin, Pfeffer, Paul E., Monopoli, Marco, Dawson, Kenneth A., Eriksson, Jonny, Edwards, Katarina, Lynham, Steven, Arno, Matthew, Behndig, Annelie F., Blomberg, Anders, Somers, Graham, Hassall, Dave, Dailey, Lea Ann, Forbes, Ben and Mudway, Ian S. (2016) Enrichment of immunoregulatory proteins in the biomolecular corona of nanoparticles within human respiratory tract lining fluid. Nanomedicine: Nanotechnology, Biology and Medicine, 12(4), pp. 1033-1043. ISSN (print) 1549-9634

Full text not available from this archive.

Abstract

When inhaled nanoparticles deposit in the lungs, they transit through respiratory tract lining fluid (RTLF) acquiring a biomolecular corona reflecting the interaction of the RTLF with the nanomaterial surface. Label-free snapshot proteomics was used to generate semi-quantitative profiles of corona proteins formed around silica (SiO2) and poly(vinyl) acetate (PVAc) nanoparticles in RTLF, the latter employed as an archetype drug delivery vehicle. The evolved PVAc corona was significantly enriched compared to that observed on SiO2 nanoparticles (698 vs. 429 proteins identified); however both coronas contained a substantial contribution from innate immunity proteins, including surfactant protein A, napsin A and complement (C1q and C3) proteins. Functional protein classification supports the hypothesis that corona formation in RTLF constitutes opsonisation, preparing particles for phagocytosis and clearance from the lungs. These data highlight how an understanding of the evolved corona is necessary for the design of inhaled nanomedicines with acceptable safety and tailored clearance profiles.

Item Type: Article
Research Area: Biological sciences
Pharmacy
Pre-clinical and human biological sciences
Faculty, School or Research Centre: Faculty of Science, Engineering and Computing (until 2017) > School of Pharmacy and Chemistry
Related URLs:
Depositing User: Anna Morgan
Date Deposited: 13 Nov 2017 16:09
Last Modified: 13 Nov 2017 16:09
DOI: https://doi.org/10.1016/j.nano.2015.12.369
URI: http://eprints.kingston.ac.uk/id/eprint/39883

Actions (Repository Editors)

Item Control Page Item Control Page