A novel method for measuring intestinal and hepatic triacylglycerol kinetics

Sun, Feifei, Stolinski, Mike, Shojaee-Moradie, Fariba, Lou, Shaoying, Ma, Yuying, Havorka, Roman and Umpleby, A. Margot (2013) A novel method for measuring intestinal and hepatic triacylglycerol kinetics. American Journal of Physiology : Endocrinology and Metabolism, 305(8), E1041-E1047. ISSN (print) 0193-1849

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Abstract

This study aimed to 1) develop a method that completely separated hepatic (VLDL1, VLDL2) and intestinal [chylomicron (CM)] lipoproteins and 2) use the method to measure triacylglycerol (TAG) kinetics in these lipoproteins in the fed and fasting state in healthy subjects, using intravenous [²H₅]glycerol as the tracer. An immunoaffinity method that completely separated hepatic and intestinal particles using sequential binding to three antibodies to apolipoprotein B-100 (apoB-100) was established and validated. Six healthy volunteers were studied in a fasted and continuous feeding study (study 1). Five additional healthy volunteers were studied in a continuous feeding study that included an oral [¹³C₃]glycerol tripalmitin tracer (study 2). In both studies, an intravenous bolus of [²H₅]glycerol was administered to label TAG in hepatic and intestinal lipoproteins. In both feeding studies there was sufficient incorporation of the [²H₅]glycerol tracer into the exogenous lipoproteins to enable isotopic enrichment to be measured. In study 2, the oral tracer enrichment in VLDL1 was <5% of CM enrichment 150 min after tracer administration, demonstrating negligible contamination of VLDL1 with apoB-48. Western blotting showed no detectable apoB-100 in CMs. VLDL1 and VLDL2 TAG fractional catabolic rate (FCR) did not differ between feeding and fasting (study 1). There was no difference between CM and VLDL1 TAG FCR in both fed studies. In fed study 2, 47% of the total TAG production rate (CM + VLDL1) was from CM. This methodology may be a useful tool for understanding the abnormalities in postprandial TAG kinetics in metabolic syndrome and type 2 diabetes.

Item Type: Article
Additional Information: This work was supported by Heart Research UK [grant number: RG 2487/04/08] and the British Heart Foundation [grant number: PG/03/157/16417].
Research Area: Biological sciences
Faculty, School or Research Centre: Faculty of Science, Engineering and Computing (until 2017) > School of Life Sciences
Related URLs:
Depositing User: Michael Stolinski
Date Deposited: 02 Nov 2017 16:35
Last Modified: 02 Nov 2017 16:35
DOI: https://doi.org/10.1152/ajpendo.00105.2013
URI: http://eprints.kingston.ac.uk/id/eprint/39237

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