Immune response of healthy adults to the ingested probiotic 'Lactobacillus casei' Shirota

Harbige, Laurence S., Pinto, Eva, Allgrove, Judith and Thomas, Linda V. (2016) Immune response of healthy adults to the ingested probiotic 'Lactobacillus casei' Shirota. Scandinavian Journal of Immunology, 84(6), pp. 353-364. ISSN (print) 0300-9475

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Abstract

Daily ingestion of a probiotic drink containing Lactobacillus casei Shirota (LcS; 1.3 x 10(10) live cells) by healthy adults for (i) 4-weeks LcS, (ii) 6-weeks discontinuation of LcS, and (iii) a final 4-weeks of LcS, was investigated. There was a significant increase in expression of the T-cell activation marker CD3(+) CD69(+) in ex vivo unstimulated blood cells at weeks 10 and 14 and a significant increase in the NK cell marker CD3(+) CD16/56(+) in ex vivo unstimulated blood cells at weeks 4, 10 and 14. Expression of the NK cell activation marker CD16/56(+) CD69(+) in ex vivo unstimulated blood cells was 62% higher at week 10 and 74% higher at week 14. Intracellular staining of IL-4 in ex vivo unstimulated and PMA/ionomycin-stimulated CD3(+) β7(+) integrin blood cells was significantly lower at week 10 and 14. Intracellular staining of IL-12 in ex vivo unstimulated and LPS-stimulated CD14(+) blood cells was significantly lower at weeks 4, 10 and 14. Intracellular staining of TNF-α in LPS-stimulated CD14(+) blood cells was significantly lower at weeks 4, 10 and 14. Mucosal salivary IFN-γ, IgA1 and IgA2 concentrations were significantly higher at week 14 but LcS did not affect systemic circulating influenza A-specific IgA or IgG and tetanus specific IgG antibody levels. In addition to the decrease in CD3(+) β7(+) integrin cell IL-4 and a CD14(+) cell anti-inflammatory cytokine profile, at week 14 increased expression of activation markers on circulating T cells and NK cells and higher mucosal salivary IgA1 and IgA2 concentration indicated a secondary boosting effect of LcS. This article is protected by copyright. All rights reserved.

Item Type: Article
Research Area: Infection and immunology
Faculty, School or Research Centre: Faculty of Science, Engineering and Computing (until 2017)
Faculty of Science, Engineering and Computing (until 2017) > School of Life Sciences
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Depositing User: Automatic Import Agent
Date Deposited: 15 Dec 2016 15:11
Last Modified: 08 Oct 2017 02:09
DOI: https://doi.org/10.1111/sji.12495
URI: http://eprints.kingston.ac.uk/id/eprint/36250

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