Potential use of ' Lactobacillus fermentum ' 3872 as an anti-campylobacter agent

Lehri, Burhan, Seddon, Alan and Karlyshev, Andrey (2016) Potential use of ' Lactobacillus fermentum ' 3872 as an anti-campylobacter agent. In: The Microbiology Society Annual Conference; 21-24 Mar 2016, Liverpool, U.K..

Full text not available from this archive.


Due to the rise of multidrug resistant form of pathogenic microorganisms, alternative intervention tools are in urgent need. One option is to employ beneficial (probiotic) bacteria, which could compete with pathogens for host cell attachment cites and elicit antibacterial activity. Lactobacillus fermentum strain 3872, which revealed outstanding probiotic activity, is a good candidate for such studies. Genome sequencing of this strain revealed a novel plasmid (pLF3872) containing a gene, encoding a collagen-binding proteins (CBP). The plasmid was not found in any other strains of the species. The chromosomal genome sequence of this strain (2.3 Mb) was found to contain other genes potentially contributing to its beneficial effects, such as those encoding a mucus binding protein and other adhesins, as well as a bacteriocin-encoding gene not found in other sequenced genomes of these bacteria. ELISA-based attachment experiments revealed competition of L. fermentum 3872 with Campylobacter jejuni strain 11168H for binding to collagen I, which is a ubiquitous structure making up the gastrointestinal tract. The results suggest that L. fermentum 3872 can potentially be used for competitive exclusion of Campylobacter jejuni, which is the most important gastrointestinal pathogen.

Item Type: Conference or Workshop Item (Poster)
Event Title: The Microbiology Society Annual Conference
Organising Body: Microbiology Society (UK)
Research Area: Allied health professions and studies
Biological sciences
Epidemiology and public health
Infection and immunology
Faculty, School or Research Centre: Faculty of Science, Engineering and Computing (until 2017) > School of Life Sciences
Depositing User: Andrey Karlyshev
Date Deposited: 07 Oct 2016 13:47
Last Modified: 07 Oct 2016 13:51
URI: http://eprints.kingston.ac.uk/id/eprint/34888

Actions (Repository Editors)

Item Control Page Item Control Page