Characterization of bone marrow mesenchymal stromal cells in aplastic anaemia.

Hamzic, Edita, Whiting, Karen, Smith, Edward Gordon and Pettengell, Ruth (2015) Characterization of bone marrow mesenchymal stromal cells in aplastic anaemia. British Journal Of Haematology, 169(6), pp. 804-813. ISSN (print) 0007-1048

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Abstract

In aplastic anaemia (AA), haemopoietic activity is significantly reduced and generally attributed to failure of haemopoietic stem cells (HSC) within the bone marrow (BM). The regulation of haemopoiesis depends on the interaction between HSC and various cells of the BM microenvironment, including mesenchymal stromal cells (MSC). MSC involvement in the functional restriction of HSC in AA is largely unknown and therefore, the physical and functional properties of AA MSC were studied in vitro. MSC were characterized by their phenotype and ability to form adherent stromal layers. The functional properties of AA MSC were assessed through proliferative, clonogenic and cross-over culture assays. Results indicate that although AA MSC presented typical morphology and distinctive mesenchymal markers, stromal formation was reduced, with 50% of BM samples failing to produce adherent layers. Furthermore, their proliferative and clonogenic capacity was markedly decreased (P = 0·03 and P = 0·04 respectively) and the ability to sustain haemopoiesis was significantly reduced, as assessed by total cell proliferation (P = 0·032 and P = 0·019 at Week 5 and 6, respectively) and clonogenic potential of HSC (P = 0·02 at Week 6). It was concluded that the biological characteristics of AA MSC are different from those of control MSC and their in vitro haemopoiesis-supporting ability is significantly reduced.

Item Type: Article
Uncontrolled Keywords: aplastic anaemia, mesenchymal cells, haematopoietic stem cells, haematopoiesis, bone marrow culture
Research Area: Biological sciences
Faculty, School or Research Centre: Faculty of Science, Engineering and Computing (until 2017)
Faculty of Science, Engineering and Computing (until 2017) > School of Life Sciences
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Depositing User: Automatic Import Agent
Date Deposited: 15 Apr 2015 10:35
Last Modified: 07 Jul 2015 09:54
DOI: https://doi.org/10.1111/bjh.13364
URI: http://eprints.kingston.ac.uk/id/eprint/31301

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