Bile salt composition is secondary to bile salt concentration in determining hydrocortisone and progesterone solubility in intestinal mimetic fluids

Zughaid, Huda, Forbes, Ben, Martin, Gary P. and Patel, Nilesh (2012) Bile salt composition is secondary to bile salt concentration in determining hydrocortisone and progesterone solubility in intestinal mimetic fluids. International Journal of Pharmaceutics, 422(1-2), pp. 295-301. ISSN (print) 0378-5173

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Abstract

Simulated intestinal fluids (SIFs) used to assay the solubility of orally administered drugs are typically based on a single bile salt; sodium taurocholate (STC). The aim of this study was to develop mimetic intestinal fluids with a closer similarity to physiological fluids than those reported to date by developing a mixed bile salt (MBS) system (STC, sodium glycodeoxycholate, sodium deoxycholate; 60:39:1) with different concentrations of lecithin, the preponderant intestinal phospholipid. Hydrocortisone and progesterone were used as model drugs to evaluate systematically the influence of SIF composition on solubility. Increasing total bile salt concentration from 0 to 30 mM increased hydrocortisone and progesterone solubility by 2- and ∼25-fold, respectively. Accordingly, higher solubilities were measured in the fed-state compared to the fasted-state SIFs. Progesterone showed the greatest increases in solubility in STC and MBS systems (2-7-fold) compared to hydrocortisone (no significant change; P>0.05) as lecithin concentration was increased. Overall, MBS systems gave similar solubility profiles to STC. In conclusion, the addenda of MBS and lecithin were found to be secondary to the influence of BS concentration. These data provide a foundation for the design of more bio-similar media for pivotal decision-guiding assays in drug development and quality control settings.

Item Type: Article
Uncontrolled Keywords: solubility, simulated intestinal fluids, hydrocortisone, progesterone, lecithin, bile salts, dissolution media, lipid formulations, biorelevant media, soluble drug, transport, micelles, lecithin, behavior, danazol, release
Research Area: Pharmacy
Faculty, School or Research Centre: Faculty of Science, Engineering and Computing (until 2017)
Faculty of Science, Engineering and Computing (until 2017) > School of Pharmacy and Chemistry
Depositing User: Davina Omar
Date Deposited: 18 Dec 2012 09:58
Last Modified: 02 Jan 2013 15:50
DOI: https://doi.org/10.1016/j.ijpharm.2011.11.012
URI: http://eprints.kingston.ac.uk/id/eprint/24395

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