The antioxidant and anti-proliferative activity of the Lebanese 'Olea europaea' extract

Fares, R, Bazzi, S, Baydoun, SE and Abdel-Massih, RM (2011) The antioxidant and anti-proliferative activity of the Lebanese 'Olea europaea' extract. Plant Foods for Human Nutrition, 66(1), pp. 58-63. ISSN (print) 0921-9668

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Abstract

It is becoming increasingly evident that certain phytochemicals possess cancer chemopreventive properties. In this study, the anti-proliferative activity of plant extracts from olive (Olea europaea L.) leaves was tested on human leukemic cell line (Jurkat). Cytotoxicity of various concentrations of plant extracts was examined and the IC(50) was determined. Olive leaf extracts showed concentration-dependent anti-proliferative effect as determined by the WST-1 proliferation kit and [(3)H]-thymidine incorporation method. To study whether cell death was due to apoptosis, cells were stained with Annexin V-FITC and PI and the expression of important regulatory proteins (Bcl-2, Bax, and p53) involved in apoptosis were examined by Western blot. The antioxidant activity of olive leaves (SC(50) = 0.1 mg dry weight) was studied using the DPPH scavenging method. Present findings suggest that olive leaves extracts exhibit anti-proliferative effect on leukemic cells by inducing apoptosis.

Item Type: Article
Faculty, School or Research Centre: Faculty of Science, Engineering and Computing (until 2017)
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Depositing User: Automatic Import Agent
Date Deposited: 15 Feb 2013 10:40
Last Modified: 15 Feb 2013 10:40
DOI: https://doi.org/10.1007/s11130-011-0213-9
URI: http://eprints.kingston.ac.uk/id/eprint/23138

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