HPV-16 E5 down-regulates expression of surface HLA class I and reduces recognition by CD8 T cells

Campo, M.S., Graham, S.V., Cortese, M.S., Ashrafi, G.H., Araibi, E.H., Dornan, E.S., Miners, K., Nunes, C. and Man, S. (2010) HPV-16 E5 down-regulates expression of surface HLA class I and reduces recognition by CD8 T cells. Virology, 407(1), pp. 137-142. ISSN (print) 0042-6822

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HPV-16 is the major causes of cervical cancer. Persistence of infection is a necessary event for progression of the infection to cancer. Among other factors, virus persistence is due the viral proteins fighting the immune response. HPV-16 E5 down-regulates MHC/HLA class I, which is much reduced on the cell surface and accumulates in the Golgi apparatus in cells expressing E5. This effect is observed also in W12 cells, which mimic early cervical intraepithelial progression to cervical cancer. The functional effect of MHC I down-regulation on human CD8 T cells is not known, because of the need for HLA-matched, HPV-specific T cells that recognise E5 expressing-cells. Here we employ a heterologous cell/MHC I system which uses mouse cells expressing both E5 and HLA-A2, and A2-restricted CTLs; we show that the E5-induced reduction of HLA-A2 has a functional impact by reducing recognition of E5 expressing cells by HPV specific CD8+ T cells.

Item Type: Article
Additional Information: The work was supported by the Association for International Cancer Research; Cancer Research UK; Cancer Research and Wales and the Libyan Ministry of Education.
Research Area: Allied health professions and studies
Cancer studies
Faculty, School or Research Centre: Faculty of Science (until 2011) > School of Life Sciences
Related URLs:
Depositing User: Gholam Ashrafi
Date Deposited: 19 Nov 2010 15:15
Last Modified: 08 Jan 2013 17:50
DOI: https://doi.org/10.1016/j.virol.2010.07.044
URI: http://eprints.kingston.ac.uk/id/eprint/17404

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