Lyophilisation and sterilisation of liposomal vaccines to produce stable and sterile products

Mohammed, Afzal R., Bramwell, Vincent W., Coombes, Allan G. A. and Perrie, Yvonne (2006) Lyophilisation and sterilisation of liposomal vaccines to produce stable and sterile products. Methods, 40(1), pp. 30-38. ISSN (print) 1046-2023

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Abstract

The advantages of liposomes as delivery systems for peptide, protein and DNA vaccines is well-recognised, unfortunately their application has been stinted by their instability during storage and their limited shelf-life. Further, sterilisation of these systems has been problematic, with degradation of the liposomes being reported after sterilisation using the various techniques available. Work form our laboratory has investigated techniques that can be applied to particulate liposomal vaccines such that they can be prepared in a freeze-dried and sterile format. In this article, we describe techniques for the lyophilisation, cryoprotection and sterilisation of liposomal vaccines. Applying these methods allows for the retention of both the chemical integrity of the lipids and the key physico-chemical characteristics of the liposomes (e.g., particle size, zeta potential, and dynamic viscosity), thus supporting the enhanced transition of liposomal vaccines from the bench to the clinic.

Item Type: Article
Uncontrolled Keywords: liposomes, freeze-drying, cyroprotectants, lyophilisation, gamma-irradiation, ionizing radiation, sterilisation, covered polystyrene microspheres, cord factor, dioctadecyldimethylammonium chloride, immunological adjuvants, colloid stability, subunit vaccines, protein, tuberculosis, vesicles, mice
Research Area: Infection and immunology
Faculty, School or Research Centre: Faculty of Science (until 2011) > School of Pharmacy and Chemistry
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Depositing User: Automatic Import Agent
Date Deposited: 29 Jan 2013 15:37
Last Modified: 01 Feb 2013 15:00
DOI: https://doi.org/10.1016/j.ymeth.2006.05.025
URI: http://eprints.kingston.ac.uk/id/eprint/13978

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