Delivery of bioactive macromolecules from microporous polymer matrices: release and activity profiles of lysozyme, collagenase and catalase

Wang, Yiwei, Chang, Hsin-I, Li, Xiongwei, Alpar, Oyar and Coombes, Allan G.A. (2009) Delivery of bioactive macromolecules from microporous polymer matrices: release and activity profiles of lysozyme, collagenase and catalase. European Journal of Pharmaceutical Sciences, 37(3-4), pp. 387-394. ISSN (print) 0928-0987

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Abstract

Microporous polycaprolactone (PCL) matrices containing lysozyme, collagenase and catalase respectively with molecular weight covering a wide range from 14.3 to 240kDa were produced by a novel method involving rapid cooling of particle suspensions in dry ice. The enzyme loading efficiency (lysozyme (50%), collagenase (75%) and catalase (90%)) depended on the enzyme molecular weight and the non-solvent used to extract acetone from the hardened matrices. Sustained enzyme release occurred from the PCL matrices over 11 days with retained activity dependent on the particular enzyme used (collagenase 100% activity at 11 days, lysozyme 75-80% at 11 days, catalase 10-20% at 5 days). The present findings confirm the potential of microporous PCL matrices for delivering bioactive macromolecules from implantable/insertable depot-type formulations and tissue engineering scaffolds and recommend catalase as a challenging model protein for evaluating such devices.

Item Type: Article
Uncontrolled Keywords: polycaprolactone, tissue engineering, scaffolds, enzymes, matrix device lysozyme, lysozyme, collagenase, catalase, enzyme activity, tissue engineering scaffolds, growth-factor, drug-delivery, protein release, stromal cells, degradation, poly(epsilon-caprolactone), polycaprolactone, microparticles, stabilization
Research Area: Pharmacy
Faculty, School or Research Centre: Faculty of Science (until 2011) > School of Pharmacy and Chemistry
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Depositing User: Susan Miles
Date Deposited: 09 Apr 2009 10:23
Last Modified: 27 May 2010 09:18
URI: http://eprints.kingston.ac.uk/id/eprint/5106

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