'Lactobacillus fermentum' 3872 as a potential tool for combatting 'Campylobacter jejuni' infections

Lehri, B., Seddon, A. M. and Karlyshev, A. V. (2017) 'Lactobacillus fermentum' 3872 as a potential tool for combatting 'Campylobacter jejuni' infections. Virulence, ISSN (print) 2150-5594 (Epub Ahead of Print)

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Abstract

Due to the global spread of multidrug resistant pathogenic bacteria, alternative approaches in combating infectious diseases are required. One such approach is the use of probiotics. Lactobacillus fermentum 3872 is a promising probiotic bacterium producing a range of antimicrobial compounds, such as hydrogen peroxide and lactic acid. In addition, previous studies involving genome sequencing and analysis of L. fermentum 3872 allowed the identification of a gene encoding a cell surface protein referred to as collagen binding protein (CBP) (not found in other strains of the species, according to the GenBank database), consisting of a C-terminal cell wall anchor domain (LPXT), multiple repeats of ‘B domains' that form stalks presenting an “A domain” required for adhesion. In this study, we found that the CBP of L. fermentum 3872 binds to collagen I present on the surface of the epithelial cells lining the gastrointestinal tract. Moreover, we found that this host receptor is also used for attachment by the major gastrointestinal pathogen, Campylobacter jejuni. Furthermore, we identified an adhesin involved in such interaction and demonstrated that both L. fermentum 3872 and its CBP can inhibit binding of this pathogen to collagen I. Combined with the observation that C. jejuni growth is affected in the acidic environment produced by L. fermentum 3872, the finding provides a good basis for further investigation of this strain as a potential tool for fighting Campylobacter infections.

Item Type: Article
Uncontrolled Keywords: adhesion, antimicrobials, Campylobacter jejuni, collagen I, collagen binding protein, Lactobacillus, multi-drug resistance, probiotics
Research Area: Agriculture, veterinary and food science
Allied health professions and studies
Biological sciences
Infection and immunology
Faculty, School or Research Centre: Faculty of Science, Engineering and Computing > School of Life Sciences
Related URLs:
Depositing User: Andrey Karlyshev
Date Deposited: 06 Sep 2017 10:52
Last Modified: 13 Oct 2017 14:08
URI: http://eprints.kingston.ac.uk/id/eprint/39152

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