Signature-tagged transposon mutagenesis identifies novel ' Mycobacterium tuberculosis ' genes involved in the parasitism of human macrophages

Rosas-Magallanes, Vania, Stadthagen-Gomez, Gustavo, Rauzier, Jean, Barreiro, Luis B., Tailleux, Ludovic, Boudou, Frederic, Griffin, Ruth, Nigou, Jerome, Jackson, Mary, Gicquel, Brigitte and Neyrolles, Olivier (2007) Signature-tagged transposon mutagenesis identifies novel ' Mycobacterium tuberculosis ' genes involved in the parasitism of human macrophages. Infection And Immunity, 75(1), pp. 504-507. ISSN (print) 0019-9567

Abstract

Using signature-tagged transposon mutagenesis, we isolated 23 Mycobacterium tuberculosis mutants, corresponding to 21 genes or genetic regions, attenuated in their ability to parasitize human macrophages. Mutants disrupted in the ABC transporter-encoding genes Rv0986 and Rv0987 were further characterized as being impaired in their ability to bind to host cells.

Actions (Repository Editors)

Item Control Page Item Control Page