New method for the analysis of heterocyclic amines in meat extracts using pressurised liquid extraction and liquid chromatography-tandem mass spectrometry

Khan, Mohammad Rizwan, Busquets, Rosa, Santos, Francisco Javier and Puignou, Lluís (2008) New method for the analysis of heterocyclic amines in meat extracts using pressurised liquid extraction and liquid chromatography-tandem mass spectrometry. Journal of Chromatography A, 1194(2), pp. 155-160. ISSN (print) 0021-9673

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Abstract

Heterocyclic amines (HAs) were analysed in meat extract samples using a new method based on pressurised liquid extraction (PLE) and liquid chromatography-tandem mass spectrometry. This method combines the use of a pressurised fluid with a triple quadrupole MS/MS system, resulting in benefits from both systems: high extraction efficiency and sensitivity. The effects of solvent type and PLE operational parameters, such as temperature and extraction time, were studied to obtain maximum recovery of the analytes with minimum contamination. HA extraction was best achieved using dichloromethane/acetone (50/50, v/v) at 80 degrees C for 10 min. Recoveries ranged from 45% to 79% with good quality parameters: limit of detection values between 0.02 and 1 ng g(-1), linearity (r(2)>0.997), and run-to-run and day-to-day precisions with relative standard deviations lower than 13% achieved at both low (0.20 microg g(-1)) and medium (1.0 microg g(-1)) concentrations. This method reduces sample manipulation and total extraction time by nearly four-fold compared to conventional solid phase extraction. The optimised method was validated using laboratory reference material based on a meat extract, and was successfully applied to HA analysis in several cooked beef samples.

Item Type: Article
Additional Information: This work was carried out with financial support from the Ministerio de Ciencia y Tecnologia, Spain, research project AGL2003-03100
Research Area: Cancer studies
Chemistry
Faculty, School or Research Centre: Faculty of Science (until 2011) > School of Pharmacy and Chemistry
Depositing User: Rosa Busquets
Date Deposited: 08 Jan 2015 11:26
Last Modified: 08 Jan 2015 11:26
URI: http://eprints.kingston.ac.uk/id/eprint/29334

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