Effect of capsule on interaction of Campylobacter jejuni cells with an analogue of a host cell receptor.

Karlyshev, Andrey, Seddon, Alan and Rubinchik, Sona (2013) Effect of capsule on interaction of Campylobacter jejuni cells with an analogue of a host cell receptor. In: 17th International Workshop on Campylobacter, Helicobacter and Related Organisms, CHRO2013; 15-19 Sep 2013, Aberdeen, United Kingdom.

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Abstract

Interaction of Campylobacter jejuni with host cells involves a variety of bacterial cell-surface structures. Whilst bacterial adhesins are required for specific binding to host cell receptors, such interaction may be impeded by production of a polysaccharide capsule. In order to elucidate a role of capsule in bacterial interaction with host cells we developed an assay based on bacterial binding to immobilised analogue of a host cell receptor, SBA lectin. The latter specifically interacts with GaNAc residues decorating N-linked glycoproteins located on the cell surface. One example of such glycoproteins, PEB3, was the main focus of this study. This model of attachment was verified in a series of experiments confirming specificity of interaction. Bacterial binding was found to be dose-dependent and affected by the presence of soluble fractions of lectin and GalNAc. Moreover, significant reduction of attachment was observed in several independent peb3 mutants. In contrast, attachment of non-capsulated kpsM was significantly increased. Expression of capsule at certain stages of infection may prevent bacterial recognition by innate host immune system, e.g. by macrophages carrying GalNAc-specific MGL receptors. On the other hand, de-protection of adhesins in non-capsulated cells may be beneficial for bacterial interaction with host tissues at the site of colonisation and attachment. A hypothesis of differential expression of adhesins and capsule was confirmed by qPCR. There was an increase of peb3 and decrease of kpsM gene expression over time. These findings suggest a role of capsule in fine tuning of C. jejuni interaction with host cells.

Item Type: Conference or Workshop Item (Poster)
Event Title: 17th International Workshop on Campylobacter, Helicobacter and Related Organisms, CHRO2013
Organising Body: Campylobacter, Helicobacter, and Related Organisms (CHRO)
Research Area: Allied health professions and studies
Biological sciences
Infection and immunology
Faculty, School or Research Centre: Faculty of Science, Engineering and Computing > School of Life Sciences
Related URLs:
Depositing User: Andrey Karlyshev
Date Deposited: 05 Nov 2013 11:25
Last Modified: 05 Nov 2013 11:25
URI: http://eprints.kingston.ac.uk/id/eprint/26692

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