A novel approach to investigating the erythroid lineage, using both receptor analysis and haemoglobin detection

McGuckin, Colin P., Liu, Wai M., Gordon-Smith, Edward C. and Uhr, Mario R. (1996) A novel approach to investigating the erythroid lineage, using both receptor analysis and haemoglobin detection. British Journal Of Haematology, 95(3), pp. 457-460. ISSN (print) 0007-1048

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Abstract

Progenitor cell failure in the erythroid lineage is a particular problem in bone marrow failure. To provide insight into early erythopoietic development we used sensitive techniques to examine the effects of SCF, IL-3 and MIP-1 alpha on two developmentally arrested progenitor cell lines, HEL and K562. Quantitative flowcytometric analysis showed that both expressed receptors (SCF > MIP-1 alpha > IL-3). Qualitative analysis revealed HEL cells expressed more receptors than K562 cells. Clonogenic assays with sensitive haemoglobin detection showed that SCF and IL-3 did not support HEL development and reduced haemoglobin production. MIP-1 alpha reduced partially developed HEL colonies and haemoglobin in developed colonies. SCF increased development, but not haemoglobin in K562 cells, with IL-3 being more effective in both. MIP-1 alpha increased the proportion of well-developed K562 colonies but not haemoglobin. This suggests SCF, IL-3 and MIP-1 alpha all have a role to play in early erythroid cellular development, with differing actions depending on the stage of development.

Item Type: Article
Uncontrolled Keywords: stem cell factor, interleukin-3, macrophage inflammatory protein-1 alpha, erythroid progenitor cells, cytokine receptors, stem-cell factor
Research Area: Allied health professions and studies
Faculty, School or Research Centre: Faculty of Science (until 2011)
Related URLs:
Depositing User: Kim Forbes
Date Deposited: 08 Aug 2008
Last Modified: 25 Nov 2011 09:52
URI: http://eprints.kingston.ac.uk/id/eprint/2491

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