Detection of testosterone and epitestosterone in human hair using liquid chromatography tandem mass spectrometry

Deshmukh, Nawed, Barker, James, Petroczi, Andrea and Naughton, Declan (2012) Detection of testosterone and epitestosterone in human hair using liquid chromatography tandem mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis, 67-68, pp. 154-158. ISSN (print) 0731-7085

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Abstract

The feasibility of using hair analysis as a complimentary test in doping control has received increased attention in the scientific community. The aim of the study was to take a step forward to this goal and develop a method that, for the first time, is able to detect testosterone (T) and epitestosterone (E) in human hair, using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and alkali digestion followed by extraction using pentane. The method was linear within the quantification range of 0.25-100pg/mg for T and 0.5-100pg/mg for E, with determination coefficient (r(2)) values >0.9987. The limits of detection for T and E were 0.1pg/mg and 0.25pg/mg respectively. The accuracy, precision and extraction recovery of the assay were satisfactory for the detection of T and E when ca. 50mg hair was processed. The validated method was successfully applied for the analysis of 75 hair samples collected from healthy volunteers (65.3% males), with the concentration of T between 0.7-11.81pg/mg and 0.33-6.05pg/mg and the concentration of E between 0.63-8.27pg/mg and 0.52-3.88pg/mg in males and in females respectively. In males, the T levels were significantly higher (p=0.020) but there was no difference in the E levels (p=0.359). However, E was not detectable in 34 samples (of which 19 were females). The T and E levels showed linear correlation (r=0.698, p<0.001) with average T/E ratio of 1.32±0.7. The newly developed analytical method was rapid, facile, sensitive, selective, reproducible and reliable for determining the levels of T and E in hair and thus for calculating the T/E ratio in hair.

Item Type: Article
Uncontrolled Keywords: human hair, testosterone, epitestosterone, liquid chromatography-tandem mass spectrometry, doping, anabolic-steroids, glucuronidation, abuse
Research Area: Allied health professions and studies
Faculty, School or Research Centre: Faculty of Science, Engineering and Computing
Faculty of Science, Engineering and Computing > School of Life Sciences
Related URLs:
Depositing User: Declan Naughton
Date Deposited: 29 May 2012 08:32
Last Modified: 26 Oct 2012 14:39
URI: http://eprints.kingston.ac.uk/id/eprint/22766

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