Baig, S., Patel, Y., Coussons, P. and Grant, R. (2001) Erythropoietin and interleukin-1[beta] modulate nitrite production in a Swiss 3T3 cell model of rheumatoid synovial fibroblasts. Biochemical Society Transactions, 30(6), pp. 883-886. ISSN (print) 0300-5127Full text not available from this archive.
Erythropoietin (EPO), a haemopoietic growth factor and a primary regulator of erythropoiesis, is widely used to treat anaemia in various chronic complications of rheumatoid arthritis (RA). Fibroblast-like cells, found in the pannus tissue of joints, are thought to contribute to the inflammatory pathology of RA. Thus for the current study we investigated the effects of recombinant human EPO (rHuEPO) on NO metabolism, using an interleukin-1beta (IL-1beta)-stimulated Swiss 3T3 fibroblast monolayer as a model for fibroblast activity in RA. The results show that, over 3 days, both alone and in combination with the pro-inflammatory cytokine IL-1beta (10 ng/ml), rHuEPO (25 micro-units/ml) induced significant production of nitrite in cell culture supernatants. This is an indicator of NO production by nitric oxide synthase (NOS), which is a well-documented mediator of metalloproteinase-mediated tissue remodelling in RA. It therefore appears that, through modulation of NOS-dependent NO production, rHuEPO may influence remodelling of connective tissue in RA, independently of its established erythropoietic role.
|Research Area:||Pre-clinical and human biological sciences|
|Faculty, School or Research Centre:||Faculty of Science (until 2011) > School of Life Sciences|
|Depositing User:||Susan Miles|
|Date Deposited:||04 Nov 2011 09:47|
|Last Modified:||04 Nov 2011 09:47|
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