Thring, Tamsyn, Hili, Pauline and Naughton, Declan (2011) Antioxidant and potential anti-inflammatory activity of extracts and formulations of white tea, rose, and witch hazel on primary human dermal fibroblast cells. Journal of Inflammation, 8, ISSN (online) 1476-9255Full text available as:
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BACKGROUND: Numerous reports have identified therapeutic roles for plants and their extracts and constituents. The aim of this study was to assess the efficacies of three plant extracts for their potential antioxidant and anti-inflammatory activity in primary human skin fibroblasts. METHODS: Aqueous extracts and formulations of white tea, witch hazel and rose were subjected to assays to measure anti-collagenase, anti-elastase, trolox equivalent and catalase activities. Skin fibroblast cells were employed to determine the effect of each extract/formulation on IL-8 release induced by the addition of hydrogen peroxide. Microscopic examination along with Neutral Red viability testing was employed to ascertain the effects of hydrogen peroxide directly on cell viability. RESULTS: Considerable anti-collagenase, anti-elastase, and antioxidant activities were measured for all extracts apart from the witch hazel distillate which showed no activity in the collagenase assay or in the trolox equivalence assay. All of the extracts and products tested elicited a significant decrease in the amount of IL-8 produced by fibroblast cells compared to the control (p < 0.05). None of the test samples exhibited catalase activity or had a significant effect on the spontaneous secretion of IL-8 in the control cells which was further corroborated with the microscopy results and the Neutral Red viability test. CONCLUSIONS: These data show that the extracts and products tested have a protective effect on fibroblast cells against hydrogen peroxide induced damage. This approach provides a potential method to evaluate the claims made for plant extracts and the products in which these extracts are found.
|Additional Information:||Article ID: 27.|
|Uncontrolled Keywords:||oxidative stress, skin-disease, elastase, plants, damage|
|Research Area:||Allied health professions and studies|
|Faculty, School or Research Centre:||Faculty of Science, Engineering and Computing
Faculty of Science, Engineering and Computing > School of Life Sciences
|Depositing User:||Declan Naughton|
|Date Deposited:||14 Nov 2011 18:57|
|Last Modified:||16 Jul 2012 21:54|
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