Moon, Alice E, Walker, Anthony J. and Goodbourn, Stephen (2011) Regulation of transcription of the Aedes albopictus cecropin A1 gene: a role for p38 mitogen-activated protein kinase. Insect Biochemistry and Molecular Biology, 41(8), pp. 628-636. ISSN (print) 0965-1748Full text not available from this archive.
Regulation of the Aedes albopictus cecropin A1 promoter was studied to provide insight into the transcriptional control of this antimicrobial peptide (AMP) gene in mosquitoes. Gene expression levels of cecropin A1 increased in A. albopictus C6/36 cells in response to heat killed Escherichiacoli. Reporter gene assays incorporating -757 to +32 of the A. albopictus cecropin A1 promoter revealed that E. coli could induce expression in these cells with more pronounced expression than that seen with lipopolysaccharide (LPS). Analysis of deletion constructs demonstrated that the 5' boundary of the regulatory region for the activation of this AMP was located between -173 and -64. Western blotting with anti-phospho-specific antibodies demonstrated that p38 mitogen-activated protein kinase (p38 MAPK) and c-Jun N-terminal kinase (JNK) were activated by LPS, whereas only p38 MAPK was activated by E. coli. Moreover, pharmacological experiments revealed that pre-incubation of cells with the p38 MAPK inhibitor SB203580 resulted in a striking activation of the cecropin A1 promoter following immune challenge, demonstrating that p38 MAPK negatively regulates cecropin A1 promoter activity. Finally the region required for the negative regulation by p38 MAPK was identified as being between -173 and -64. This report is the first to show involvement of the p38 MAPK pathway in the negative regulation of AMP production in a mosquito.
|Uncontrolled Keywords:||mosquito defence, antimicrobial peptides, promoter, c6/36 cells, p38 mapk, innate immunity, nf-kappa-b, mosquito cell-line, distinct signaling pathways, tumor-necrosis-factor, genome-wide analysis, anopheles-gambiae, immune-response, medfly hemocytes, Drosophila, Aegypti|
|Research Area:||Biological sciences|
|Faculty, School or Research Centre:||Faculty of Science (until 2011) > School of Life Sciences|
|Depositing User:||Automatic Import Agent|
|Date Deposited:||11 May 2011 13:54|
|Last Modified:||16 Nov 2011 16:18|
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