Application of methionine as a detector molecule for the assessment of oxygen radical generation by human neutrophils and endothelial cells.

Stevens, C, Bucurenci, N, Abbot, S, Sahinoglu, T, Blake, D, Naughton, D and Grootveld, M (1992) Application of methionine as a detector molecule for the assessment of oxygen radical generation by human neutrophils and endothelial cells. Free Radical Research Commun., 17(2), pp. 143-154. ISSN (print) 1071-5762

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Abstract

Diverse cell types can generate reactive oxygen species (ROS) which are implicated in many disease processes and are ascribed both beneficial and deleterious roles. In vitro studies of this phenomenon indicate that properties of the microenvironment in culture influence the cells' behaviour with regard to ROS generation in vivo. To date, however, the assessment of cellular ROS generation has been limited to techniques which are invasive of the culture environment, or require cells to be in suspension. This study describes the application of NMR spectroscopy to the detection of ROS generation, a technique which is non-invasive of the cell culturing environment.

Item Type: Article
Research Area: Allied health professions and studies
Faculty, School or Research Centre: Faculty of Science (until 2011) > School of Life Sciences
Related URLs:
Depositing User: Declan Naughton
Date Deposited: 07 Feb 2012 12:22
Last Modified: 05 Mar 2013 11:15
URI: http://eprints.kingston.ac.uk/id/eprint/18237

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