Generation of substance P carbamate in neutral aqueous solution : relevance to inflammatory joint diseases

Davis, Adrian J., Hawkes , Geoffrey E., Hatcock, Peter R., O'Brien, Paul, Kidd, Bruce L., Mapp, Paul I., Naughton, Declan and Grootveld, Martin (1993) Generation of substance P carbamate in neutral aqueous solution : relevance to inflammatory joint diseases. FEBS Letters, 329(3), pp. 249-252. ISSN (print) 0014-5793

Full text not available from this archive.

Abstract

High-field proton (1H) nuclear magnetic resonance (NMR) spectroscopy has been employed to evaluate the formation of substance P carbamate in aqueous solution. Equilibration of substance P with physiologically relevant concentrations of bicarbonate (2.50 x 10(-2) mol.dm-3) at pH 7.00 generated a new multiplet signal centred at 4.13 ppm in its NMR spectrum, characteristic of the alpha-proton of peptide carbamate species. High-field 1H NMR spectroscopy also demonstrated that the model dipeptide, Arg-Gly, formed a carbamate in neutral aqueous solutions containing 2.50 x 10(-2) mol.dm-3 HCO3-. The physiological significance of these results is discussed in view of the central roles of vasoactive neuropeptides in human joint diseases and the hypercapnic environment of the inflamed rheumatoid joint.

Item Type: Article
Additional Information: This work was supported by the Arthritis and Rheumatism Council (UK).
Research Area: Allied health professions and studies
Faculty, School or Research Centre: Faculty of Science (until 2011) > School of Life Sciences
Related URLs:
Depositing User: Declan Naughton
Date Deposited: 28 Feb 2012 10:07
Last Modified: 28 Feb 2012 10:07
URI: http://eprints.kingston.ac.uk/id/eprint/18233

Actions (Repository Editors)

Item Control Page Item Control Page