Davis, Adrian J., Hawkes , Geoffrey E., Hatcock, Peter R., O'Brien, Paul, Kidd, Bruce L., Mapp, Paul I., Naughton, Declan and Grootveld, Martin (1993) Generation of substance P carbamate in neutral aqueous solution : relevance to inflammatory joint diseases. FEBS Letters, 329(3), pp. 249-252. ISSN (print) 0014-5793Full text not available from this archive.
High-field proton (1H) nuclear magnetic resonance (NMR) spectroscopy has been employed to evaluate the formation of substance P carbamate in aqueous solution. Equilibration of substance P with physiologically relevant concentrations of bicarbonate (2.50 x 10(-2) mol.dm-3) at pH 7.00 generated a new multiplet signal centred at 4.13 ppm in its NMR spectrum, characteristic of the alpha-proton of peptide carbamate species. High-field 1H NMR spectroscopy also demonstrated that the model dipeptide, Arg-Gly, formed a carbamate in neutral aqueous solutions containing 2.50 x 10(-2) mol.dm-3 HCO3-. The physiological significance of these results is discussed in view of the central roles of vasoactive neuropeptides in human joint diseases and the hypercapnic environment of the inflamed rheumatoid joint.
|Additional Information:||This work was supported by the Arthritis and Rheumatism Council (UK).|
|Research Area:||Allied health professions and studies|
|Faculty, School or Research Centre:||Faculty of Science (until 2011) > School of Life Sciences|
|Depositing User:||Declan Naughton|
|Date Deposited:||28 Feb 2012 10:07|
|Last Modified:||28 Feb 2012 10:07|
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