Homer, K. A., Grootveld, M. C., Hawkes, J., Naughton, D.P. and Beighton, D. (1994) Degradation of hyaluronate by Streptococcus intermedius strain UNS 35. Journal of Medical Microbiology, 41(6), pp. 414-422. ISSN (print) 0022-2615
Full text not available from this archive.Abstract
Streptococcus intermedius strain UNS 35, a brain abscess isolate, produced extracellular hyaluronidase when grown in brain heart infusion broth. Chemical assays with this enzyme indicated that hyaluronate depolymerisation resulted in the formation of carbohydrate moieties with N-acetylglucosamine at the reducing terminal and containing an unsaturated carbon-carbon double bond. The nature of the products of this hyaluronidase were investigated further by high-field (400 MHz) proton (1H) NMR spectroscopy. Treatment of hyaluronate with the enzyme resulted in a series of new, sharp resonances in spectra (acetamido methyl group singlets located at 2.03 and 2.07 ppm, sugar ring proton multiplets in the 3.5-4.2 ppm chemical shift range, and doublets at 5.16 and 5.87 ppm) characteristic of low-M(r) oligosaccharide species, predominantly those containing glucuronosyl residues with delta 4,5-carbon-carbon double bonds. Comparison of spectra acquired from hyaluronidase-treated samples with that of an authentic sample of 4-deoxy-L-threo-hex-4-enopyranosyluronic-acid-N-acetylglucosamine (delta UA GlcNAc) indicated that this disaccharide was a major product arising from the actions of this enzyme. When used in minimal media, hyaluronate supported growth of S. intermedius, with lactate as the major metabolic end-product.
| Item Type: | Article |
|---|---|
| Additional Information: | This work was supported by the Arthritis and Rheumatisim Research Council (UK). |
| Research Area: | Allied health professions and studies |
| Faculty, School or Research Centre: | Faculty of Science (until 2011) > School of Life Sciences |
| Depositing User: | Declan Naughton |
| Date Deposited: | 23 Apr 2010 11:06 |
| Last Modified: | 17 Feb 2011 16:17 |
| URI: | http://eprints.kingston.ac.uk/id/eprint/11906 |
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