Insect infection model for Campylobacter jejuni reveals that O-methyl phosphoramidate has insecticidal activity

Champion, Olivia L, Karlyshev, Andrey V, Senior, Nicola J, Woodward, Martin, La Ragione, Roberto, Howard, Sarah L, Wren, Brendan W and Titball, Richard W (2010) Insect infection model for Campylobacter jejuni reveals that O-methyl phosphoramidate has insecticidal activity. The Journal of Infectious Diseases, 201(5), pp. 776-782. ISSN (print) 0022-1899

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Abstract

Galleria mellonella (wax moth) larvae have elsewhere been shown to be susceptible to pathogens such as Francisella tularensis, Burkholderia mallei, and Pseudomonas aeruginosa. We report that the larvae are rapidly killed by Campylobacter jejuni at 37 degrees C. Three strains of C. jejuni tested, 11168H (human diarrheal isolate), G1 (human Guillain-Barré syndrome isolate), and 81-176 (human diarrheal isolate), were equally effective at killing G. mellonella larvae. A panel of defined mutants of C. jejuni 11168H, in known or putative virulence genes, showed different degrees of attenuation in G. mellonella larvae. A mutant lacking the O-methyl phosphoramidate (MeOPN) capsule side group was attenuated, clearly demonstrating that MeOPN has a role in virulence. This new model of C. jejuni infection should facilitate the identification of novel virulence genes.

Item Type: Article
Research Area: Biological sciences
Faculty, School or Research Centre: Faculty of Science (until 2011) > School of Life Sciences
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Depositing User: Automatic Import Agent
Date Deposited: 27 May 2010 10:09
Last Modified: 08 Feb 2013 15:21
URI: http://eprints.kingston.ac.uk/id/eprint/11856

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